REPLICA PLATING AND INDIRECT SELECTION OF BACTERIAL MUTANTS

Replica plating and indirect selection of bacterial mutants: isolation of preadaptive mutants in bacteria by sib selection.

A modified device for replica plating.

409 repeated centrifugation and/or a series of sieving steps N0rgaard described a technique utilizing density gradient centrifugation. Although high purity and ultrastructural preservation were seen, the procedure required renal perfusion with a special medium, and yields of pure glomeruli were relatively low (10 to 20%). The current procedure is simple and rapid and requires neither renal perf...

Plasmid transformation and replica filter plating of Acholeplasma laidlawii.

The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin. Transformed primary colonies were easily recognized due to a different colon...

Evaluation of "floc" as a Replica Plating Material for Bacteria.

and spread during the summer to widely separated locations. In addition to white perch (Roccus americanus), striped bass (R. saxatilis) were involved to a lesser degree. Carefully selected moribund specimens were collected and delivered to the laboratory. Microscopy disclosed an abundance of polarly staining gram-negative rods in blood and organs of diseased fish. First isolations of these bact...

Determination of carbon assimilation patterns of yeasts by replica plating.

For the differentiation of species of yeasts, considerable value is attached to the ability of aimilating various carbon sources for growth. Beijerinck (1889) introduced for this general purpose the auxanographic plate technique which was adapted by Lodder (1934) for the study of sugar assimilation in the nonfermenting strains of the asporogenous yeasts. Diddens and Lodder (1942) expanded its u...